You Position: Home > Paper
Oligonucleotide uptake in hematological tumor cells is related to cellular species and proliferation
( views:166, downloads:1 )
- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0513-4870.2003.06.001
- Key Word:
- 反义寡核苷酸;白血病;淋巴瘤;bcl-2基因
Abstract： 目的探讨血液肿瘤细胞摄取寡核苷酸与其种类和活性的关系.方法流式细胞仪测定细胞内的平均荧光强度.结果经过0.60 μmol*L-1 荧光素FITC标记的G3139作用4 h后,血液系统肿瘤患者外周血及骨髓单个核细胞对G3139的摄取能力明显高于正常人;不同来源的血液肿瘤细胞株摄取G3139的能力不同,单核细胞、B淋巴细胞和髓系粒细胞来源的白血病细胞的摄取能力明显高于T淋巴细胞来源的白血病细胞;经过全反式维甲酸作用的HL60细胞的增殖活性明显受到抑制,同时细胞摄取G3139的能力明显下降.结论血液肿瘤细胞具有摄取寡核苷酸的能力,这种摄取能力与其细胞种类和细胞增殖活性有关.
- [1]朱元贵,卓光生,陈志哲,等.阳离子脂质体增加bcl-2反义寡脱氧核苷酸G3139在HL-60细胞中的摄取及活性[J].2001,(11).
- [2]W Y, Gao,J W, Jaroszewski,J S, Cohen,etc.Mechanisms of inhibition of herpes simplex virus type 2 growth by 28-mer phosphorothioate oligodeoxycytidine.[J].1990,265(33).
- [3]B, Calabretta,R B, Sims,M, Valtieri,etc.Normal and leukemic hematopoietic cells manifest differential sensitivity to inhibitory effects of c-myb antisense oligodeoxynucleotides: an in vitro study relevant to bone marrow purging.[J].1991,88.
- [4]RICHARD I. HOGREFE.An Antisense Oligonucleotide Primer[J].1999,9(4).
- [5]Q, Zhao,X, Song,T, Waldschmidt,etc.Oligonucleotide uptake in human hematopoietic cells is increased in leukemia and is related to cellular activation.[J].1996,88(5).
- [6]Lancashire H,Orr RM,Beck T,etc.Pharmacokinetics of G3139, a phosphorothioate oligodeoxynucleotide antisense to bcl-2, after intravenous administration or continuous subcutaneous infusion to mice.[J].1997,281(1).
- [7]朱元贵,卓光生.脂质体转染bcl-2反义寡核苷酸G3139在HL-60细胞中的动力?…[J].2000,016 (5).
- [8]R, Kronenwett,U, Steidl,M, Kirsch,etc.Oligodeoxyribonucleotide uptake in primary human hematopoietic cells is enhanced by cationic lipids and depends on the hematopoietic cell subset.[J].1998,91(3).