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Effects of sodium magnesium fructose diphosphate on free calcium concentration and nitric oxide synthase activity of ischemic synaptosome

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Author:
No author available
Journal Title:
ACTA PHARMACEUTICA SINICA
Issue:
5
DOI:
10.3321/j.issn:0513-4870.2003.05.002
Key Word:
脑缺血;果糖二磷酸钠镁;1,6-二磷酸果糖;突触体;游离钙;一氧化氮合酶;cerebral ischemia;sodium magnesium fructose diphosphate;fructose-1,6-diphosphate;synaptosome;free calcium;nitric oxide synthase

Abstract: Aim To study the effects of sodium magnesium fructose diphosphate (SMFD) on free calcium concentration and nitric oxide synthase activity of ischemic synaptosome, so as to explore the protective mechanisms of SMFD on cerebral ischemia. Methods The synaptosomes from normal rat brain were prepared by phase partition and cultured with oxygen-glucose deprivation to establish ischemic synaptosome model. The intrasynaptosomal free calcium concentration and nitric oxide synthase activity were detected separtately after the synaptosomes were co-incubated with SMFD (1.3 mmol*L-1) or fructose-1,6-diphosphate (FDP, 4.0 mmol*L-1) for 60 min. Results SMFD decreased the free calcium concentration and reduced the activity of nitric oxide synthase (NOS) of ischemic synaptosomes. Its effects were more powerful than those of FDP. Conclusion SMFD may protect neurons from ischemic injury by preventing intracellular Ca2+ overload and inhibiting the activity of nitric oxide synthase.

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