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Effect of lipopolysaccharide on expression of matrix metalloproteinase-9 in human synoviocyte from patients with rheumatoid arthritis
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 4
- DOI:
- 10.3321/j.issn:0513-4870.2003.04.002
- Key Word:
- 成纤维状滑膜细胞;U937细胞;基质金属蛋白酶-9;脂多糖;地塞米松
Abstract： 目的研究脂多糖(LPS)对人类风湿性关节炎(RA)成纤维状滑膜细胞(FLS)基质金属蛋白酶-9(MMP-9)表达的影响.方法明胶酶谱法测定MMP-9酶活性;Western blot法测定MMP-9蛋白的表达;RT-PCR法测定MMP-9 mRNA的表达.结果 LPS处理对FLS中MMP-9表达无显著影响;LPS刺激的U937细胞培养上清液可明显增强FLS 中MMP-9酶活性、蛋白分泌及mRNA表达;地塞米松可显著抑制上述变化,且其抑制作用随浓度的增加而增强.结论 LPS对FLS中MMP-9表达无直接影响,LPS刺激的U937细胞上清液使FLS中MMP-9表达增加,而地塞米松能抑制MMP-9的变化.
- [1]李良成,侯琦,程桂芳,等.大鼠中性粒细胞与滑膜细胞粘附模型的建立[J].2000,(2).
- [2]李怡棠,刘柏合,张成义,等.地塞米松和吲哚美辛对基质金属蛋白酶-9的抑制作用及其机制研究[J].2003,(1).
- [3]T M, Leber,F R, Balkwill.Zymography: a single-step staining method for quantitation of proteolytic activity on substrate gels.[J].1997,249(1).
- [4]Diane Ahrens,Alisa E. Koch,Richard M. Pope,etc.Expression of matrix metalloproteinase 9 (96-kd gelatinase B) in human rheumatoid arthritis[J].1996,39(9).
- [5]U K, Saarialho-Kere,H G, Welgus,W C, Parks.Distinct mechanisms regulate interstitial collagenase and 92-kDa gelatinase expression in human monocytic-like cells exposed to bacterial endotoxin.[J].1993,268(23).
- [6]C, Ries,H, Kolb,P E, Petrides.Regulation of 92-kD gelatinase release in HL-60 leukemia cells: tumor necrosis factor-alpha as an autocrine stimulus for basal- and phorbol ester-induced secretion.[J].1994,83(12).
- [7]J G, Tebib.Apoptosis: relevance to rheumatology.[J].1995,62(10).
- [8]A. Hui,G. V. Kulkarni,W. L. Hunter,etc.Paclitaxel selectively induces mitotic arrest and apoptosis in proliferating bovine synoviocytes[J].1997,40(6).
- [9]M P, Vincenti,I M, Clark,C E, Brinckerhoff.Using inhibitors of metalloproteinases to treat arthritis. Easier said than done?[J].1994,37(8).
- [10]B A, Osborne.Apoptosis and the maintenance of homoeostasis in the immune system.[J].1996,8(2).