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Ginsenoside Rg1 may protect SHSY5Y cells from apoptosis induced by MPP+ through JNK way
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 3
- DOI:
- 10.3321/j.issn:0513-4870.2003.03.005
- Key Word:
- 人参皂苷;SHSY5Y细胞;细胞凋亡;丝裂素活化蛋白激酶
Abstract： 目的探讨人参皂苷Rg1对MPP+诱导细胞凋亡保护作用的可能信号传导途径.方法用吖啶橙-溴化乙锭染色观察SHSY5Y细胞凋亡率,流式细胞仪检测细胞内活性氧ROS水平,Western Blotting法检测JNK(c-jun NH2-terminal kinase)激酶活性,免疫细胞化学染色法检测裂解的Caspase-3阳性细胞的表达率.结果经10 μmol*L-1 Rg1或2.5 mmol*L-1 N-乙酰半胱氨酸预处理后,MPP+诱导的SHSY5Y细胞凋亡受到明显抑制,同时细胞内ROS下降,JNK激酶的活性减弱,裂解的Caspase-3阳性细胞表达率下降.结论 Rg1可抑制MPP+诱导的SHSY5Y细胞凋亡,其作用机制可能是通过清除ROS、减弱JNK激酶的活性,从而减少Caspase-3的激活.
- [1]方芳,陈晓春,朱元贵.氧化应激是MPP+诱导SHSY5Y细胞凋亡的可能机制[J].2002,(2).
- [2]陈晓春,朱元贵,王小众,等.人参皂苷Rg1对PC12细胞凋亡保护作用的可能机制[J].2001,(6).
- [3]陈晓春,朱元贵,王小众,等.人参皂甙Rg1对抗多巴胺诱导的PC12细胞凋亡1[J].2001,(8).
- [4]Saporito MS,Brown EM,Carswell S,etc.CEP-1347/KT-7515, an inhibitor of c-jun N-terminal kinase activation, attenuates the 1-methyl-4-phenyl tetrahydropyridine-mediated loss of nigrostriatal dopaminergic neurons In vivo.[J].1999,288(2).
- [5]M, Verheij,R, Bose,X H, Lin,etc.Requirement for ceramide-initiated SAPK/JNK signalling in stress-induced apoptosis.[J].1996,380(6569).
- [6]J T, Zhang.[Retrospect and prospect of ginseng research].[J].1995,30(5).
- [7]W. Davis Parker Jr,Russell H. Swerdlow,Janice P. Parks,etc.Elevated reactive oxygen species and antioxidant enzyme activities in animal and cellular models of Parkinson's disease[J].1997,1362(1).
- [8]Emily H.-Y. Cheng,David G. Kirsch,Rollie J. Clem,etc.Conversion of Bc1-2 to a Bax-like Death Effector by Caspases[J].1997,278(5345).
- [9]李君庆,张香阁,张均田.人参皂甙Rg1抗神经细胞凋亡作用机制的研究[J].1997,(6).