You Position: Home > Paper
Protective effect of losartan on bovine cerebral microvessel endothelial cell injury induced by angiotensin II in culture
( views:196, downloads:5 )
- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0513-4870.2003.01.002
- Key Word:
- 脑微血管内皮细胞;血管紧张素II;氯沙坦;AT1受体
Abstract： 目的观察氯沙坦对血管紧张素II(Ang II)致牛脑微血管内皮细胞(BCMECs)损伤的保护作用.方法用分光光度计测定培养的BCMECs乳酸脱氢酶(LDH)的漏出量,流式细胞仪测定BCMECs细胞间粘附分子-1(ICAM-1)的表达量,硝酸还原酶法和放射免疫分析法分别测定BCMECs上清液中一氧化氮(NO)和内皮素-1(ET1)的含量.结果 Ang II呈剂量依赖性增加BCMECs LDH漏出、NO和ET1释放及ICAM-1表达,氯沙坦对此均有明显抑制作用.结论氯沙坦抑制Ang II致体外培养BCMECs的损伤.
- [1]P, Fornes,C, Richer,E, Vacher,etc.Losartan's protective effects in stroke-prone spontaneously hypertensive rats persist durably after treatment withdrawal.[J].1993,22(2).
- [2]Y, Numagami,A B, Zubrow,O P, Mishra,etc.Lipid free radical generation and brain cell membrane alteration following nitric oxide synthase inhibition during cerebral hypoxia in the newborn piglet.[J].1997,69(4).
- [3]Rose JM,Audus KL.AT1 receptors mediate angiotensin II uptake and transport by bovine brain microvessel endothelial cells in primary culture.[J].1999,33(1).
- [4]M S, Diamond,D E, Staunton,S D, Marlin,etc.Binding of the integrin Mac-1 (CD11b/CD18) to the third immunoglobulin-like domain of ICAM-1 (CD54) and its regulation by glycosylation.[J].1991,65(6).
- [5]M E, Pueyo,W, Gonzalez,A, Nicoletti,etc.Angiotensin II stimulates endothelial vascular cell adhesion molecule-1 via nuclear factor-kappaB activation induced by intracellular oxidative stress.[J].2000,20(3).
- [6]周武,于步润.脑梗塞患者血浆内皮素与肾素-血管紧张素系统及降钙素基因相关肽的动态观察[J].1997,(3).
- [7]S, Saito,Y, Hirata,T, Emori,etc.Angiotensin II activates endothelial constitutive nitric oxide synthase via AT1 receptors.[J].1996,19(3).
- [8]Ziv I.,Fleminger G.,Djaldetti R.,etc.Increased plasma endothelin-1 in acute ischemic stroke[J].1992.
- [9]Michel JB,Pueyo ME,Rami J,etc.Angiotensin II stimulates the production of NO and peroxynitrite in endothelial cells.[J].1998,274(1 Pt.1).
- [10]H, Ito,K, Takemori,J, Kawai,etc.AT1 receptor antagonist prevents brain edema without lowering blood pressure.[J].2000,76.
- [11]C, Page,M, Rose,M, Yacoub,etc.Antigenic heterogeneity of vascular endothelium.[J].1992,141(3).