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EFFECT OF SALIDROSIDE ON THE PROLIFERATION AND COLLAGEN mRNA TRANSCRIPTION IN RAT HEPATIC STELLATE CELLS STIMULATED BY ACETALDEHYDE
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 11
- DOI:
- 10.3321/j.issn:0513-4870.2002.11.003
- Key Word:
- 红景天苷;乙醛;肝星状细胞;细胞增殖;胶原基因
Abstract： 目的探讨红景天苷对乙醛刺激的大鼠肝星状细胞(hepatic stellate cell,HSC)增殖、α1(I)型胶原mRNA合成、IκB和NF-κB表达的影响.方法用原位杂交、免疫细胞化学和凝胶电泳迁移率技术(EMSA)检测HSC的增殖和胶原基因表达.结果乙醛刺激HSC增殖、α1(I)型胶原mRNA合成 ,使IκB表达下降、NF-κB活性增加.红景天苷(0.5～2.5 mg*mL-1)对上述影响有抑制作用,且以1.5 mg*mL-1组效果最好.结论红景天苷明显抑制乙醛刺激的HSC增殖及胶原基因的表达.
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