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SODIUM SELENITE-INDUCED OXIDATIVE STRESS AND APOPTOSIS IN HUMAN ACUTE PROMYELOCYTIC LEUKEMIA NB4 CELLS
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 9
- DOI:
- 10.3321/j.issn:0513-4870.2002.09.002
- Key Word:
- 硒;急性早幼粒细胞白血病;氧化应激;细胞凋亡
Abstract： 目的研究亚硒酸钠诱导NB4细胞的氧化应激和细胞凋亡.方法 MTT比色法检测亚硒酸钠对NB4细胞的生长抑制;用形态学、DNA琼脂糖电泳和流式细胞术研究亚硒酸钠诱导NB4细胞凋亡的作用;用化学发光法和比色法研究亚硒酸钠对NB4细胞内氧化应激的影响. 结果亚硒酸钠可以时间和剂量依赖性地抑制NB4细胞生长和诱导NB4细胞凋亡.亚硒酸钠(≥5 μmol*L-1)提高了NB4细胞内ROS水平,同时伴有细胞内还原型谷胱甘肽含量下降,而抗氧化剂NAC可抑制亚硒酸钠诱导的NB4细胞氧化应激和细胞凋亡.结论亚硒酸钠诱导NB4细胞氧化应激可能是其诱导NB4细胞凋亡的重要机理.
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