You Position: Home > Paper
STUDIES ON EVODIAMINE INDUCED HELA CELL APOPTOSIS
( views:60, downloads:8 )
- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 9
- DOI:
- 10.3321/j.issn:0513-4870.2002.09.001
- Key Word:
- 吴茱萸碱;HeLa细胞;细胞凋亡
Abstract： 目的研究吴茱萸碱(evodiamine)诱导人子宫颈癌HeLa细胞凋亡的分子生物学机制.方法用结晶紫法和琼脂糖凝胶电泳法以及用两种caspase的蛋白酶抑制剂测定细胞凋亡过程中caspase的信号传导路径.结果吴茱萸碱可诱导HeLa细胞的细胞膜皱缩、细胞质浓集及凋亡小体的形式,并清晰可见以180 bp倍数裂解的DNA梯形电泳带的出现.抑制剂VAD-fmk(caspase家族总抑制剂)和DEVD-fmk(caspase-3抑制剂)能部分抑制HeLa细胞的凋亡.结论 Evodiamine诱导人宫颈癌HeLa细胞凋亡;caspase cascade信号传导路径与凋亡密切相关.
- [1]Earnshaw WC.,Kaufmann SH.,Villa P..CASPASES AND CASPASE INHIBITORS [Review][J].1997,22(10).
- [2]H, Steller.Mechanisms and genes of cellular suicide.[J].1995,267(5203).
- [3]Takahashi A,Earnshaw WC.Ice-Related Proteases in Apoptosis[J].1996,6(1).
- [4]Wen-Fei Chiou,Cheng-Jen Chou,Andrew Yau-Chik Shum,etc.The vasorelaxant effect of evodiamine in rat isolated mesenteric arteries: mode of action[J].1992,215(2-3).
- [5]G S, Salvesen,V M, Dixit.Caspases: intracellular signaling by proteolysis.[J].1997,91(4).
- [6]Wen-Fei Chiou,Yen-Jen Sung,Jyh-Fei Liao,etc.Inhibitory Effect of Dehydroevodiamine and Evodiamine on Nitric Oxide Production in Cultured Murine Macrophages[J].1997,60(7).
- [7]J, Yamahara,T, Yamada,T, Kitani,etc.Antianoxic action of evodiamine, an alkaloid in Evodia rutaecarpa fruit.[J].1989,27(1-2).
- [8]Masaru Ogasawara,Toshiyuki Matsubara,Hideyo Suzuki.Inhibitory Effects of Evodiamine on in Vitro Invasion and Experimental Lung Metastasis of Murine Colon Cancer Cells[J].2001,24(8).
- [9]M, Enari,R V, Talanian,W W, Wong,etc.Sequential activation of ICE-like and CPP32-like proteases during Fas-mediated apoptosis.[J].1996,380(6576).
- [10]Rao L,White E.Bcl-2 and the ICE family of apoptotic regulators: making a connection[J].1997,7(1).
- [11]Nakano Y,Hoshikuma K,Kobayashi Y,etc.The bronchoconstrictive action of evodiamine, an indoloquinazoline alkaloid isolated from the fruits of Evodia rutaecarpa, on guinea-pig isolated bronchus: possible involvement on vanilloid receptors.[J].2000,66(6).
- [12]Masaru Ogasawara,Toshiyuki Matsubara,Hideyo Suzuki.Screening of Natural Compounds for Inhibitory activity on Colon Cancer Cell Migration[J].2001,24(6).
- [13]M, Yoshizumi,H, Houchi,Y, Ishimura,etc.Effect of evodiamine on catecholamine secretion from bovine adrenal medulla.[J].1997,44.