You Position: Home > Paper
mRNA EXPRESSION OF MUSCARINIC RECEPTORS IN SPINAL CORD AND BRAINSTEM IN MORPHINE DEPENDENT RATS
( views:135, downloads:5 )
- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 8
- DOI:
- 10.3321/j.issn:0513-4870.2002.08.005
- Key Word:
- 毒蕈碱受体;吗啡依赖;东莨菪碱;阿片受体;戒断症状
Abstract： 目的观察吗啡依赖大鼠脊髓和脑干毒蕈碱型乙酰胆碱受体m1～5的表达.方法以β-actin为内标,用RT-PCR方法检测m1～5的表达.结果吗啡依赖大鼠脊髓m1～5受体和脑干中m1和m2表达较正常对照组明显升高,注射纳洛酮1 h后脊髓m1～4和脑干m1表达较依赖组减少.东莨菪碱(0.5 mg*kg-1,ip)或呱伦西平(10 mg*kg-1,ip)明显减少吗啡戒断症状,呱伦西平处理后脊髓m1,m2,m3和m5表达较戒断对照组增加,东莨菪碱处理后脊髓m2,m3和m4表达增加.结论脊髓M受体适应性改变是吗啡戒断症状表达的生物学基础.
- [1]C F, Liao,A P, Themmen,R, Joho,etc.Molecular cloning and expression of a fifth muscarinic acetylcholine receptor.[J].1989,264(13).
- [2]G, Yang,K, Xu,Q, Luo.[Clinical study of scopolamine detoxification for the treatment of heroin addicts].[J].1996,76(2).
- [3]Roopra A,Buckley NJ,Wood IC,etc.Structure of the m4 cholinergic muscarinic receptor gene and its promoter.[J].1995,270(52).
- [4]Zhang, L. C.,Buccafusco, J. J..Prevention of morphine-induced muscarinic (M2) receptor adaptation suppresses the expression of withdrawal symptoms[J].1998,803(1).
- [5]Klett CP,Bonner TI.Identification and characterization of the rat M1 muscarinic receptor promoter.[J].1999,72(3).
- [6]Carla Ghelardini,Nicoletta Galeotti,Alessandro Bartolini.Loss of muscarinic antinociception by antisense inhibition of M1 receptors[J].2000,129(8).
- [7]F, Fukamauchi,P A, Saunders,C, Hough,etc.Agonist-induced down-regulation and antagonist-induced up-regulation of m2- and m3-muscarinic acetylcholine receptor mRNA and protein in cultured cerebellar granule cells.[J].1993,44(5).
- [8]L.N. Holland,L.C. Shuster,J.J. Buccafusco.Role of spinal and supraspinal muscarinic receptors in the expression of morphine withdrawal symptoms in the rat[J].1993,32(12).
- [9]J, Gocayne,D A, Robinson,M G, FitzGerald,etc.Primary structure of rat cardiac beta-adrenergic and muscarinic cholinergic receptors obtained by automated DNA sequence analysis: further evidence for a multigene family.[J].1987,84.
- [10]TI Bonner,NJ Buckley,AC Young,etc.Identification of a family of muscarinic acetylcholine receptor genes[J].1987,237 (4814).
- [11]Hoglund AU,Baghdoyan HA.M2, M3 and M4, but not M1, muscarinic receptor subtypes are present in rat spinal cord.[J].1997,281(1).