You Position: Home > Paper
CAPILLARY ZONE ELECTROPHORESIS FOR DETERMINATION OF THYMULIN IN HUMAN PLASMA
( views:119, downloads:8 )
- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 3
- DOI:
- 10.3321/j.issn:0513-4870.2002.03.015
- Key Word:
- 胸腺九肽;毛细管电泳法;人血浆
Abstract： 目的建立定量测定人血浆中胸腺九肽的毛细管区带电泳法.方法运行缓冲液为50 mmol·L-1 Na2B4O7-10 mmol·L-1 Na2HPO4 (pH 9.10).毛细管总长57 cm,有效长度50 cm,内径75 μm.运行电压12 kV,运行温度25℃.检测波长:200 nm.结果胸腺九肽的线性范围为1～10 μg·mL-1,γ=0.9990,重现性RSD为3.05%,回收率为70.97%,RSD为3.32%.结论毛细管电泳法可检测人血浆胸腺九肽的含量,方法简便、可靠、快速.
- [1]曲宁,陈竞,夏弈明.毛细管区带电泳测定血清胸腺因子的方法[J].2001,(5).
- [2]Mireille Dardenne,Wilson Savino,Sali Wade,etc.In vivoandin vitrostudies of thymulin in marginally zinc-deficient mice[J].1984,14(5).