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PROTECTIVE EFFECTS OF TACRINE AND DONEPEZIL AGAINST STAUROSPORINE-INDUCED APOPTOTIC DEATH
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 2
- DOI:
- 10.3321/j.issn:0513-4870.2002.02.005
- Key Word:
- 他克林;多奈哌齐;乙酰胆碱酯酶抑制剂;细胞凋亡;阿尔茨海默病
Abstract： 目的用星形孢菌素(STS)诱导NG108-15和HeLa细胞凋亡,观察他克林和多奈哌齐是否具有抗凋亡作用.方法MTT测定分析细胞损伤状况;相差显微镜和Hoechst 33342染色观察细胞及胞核形态;DNA提取及琼脂糖凝胶电泳观察凋亡特征性梯带;免疫印迹分析Bcl-2和Bax的表达水平.结果经0.1 mmol·L-1他克林预处理后,由STS诱导的NG108-15细胞凋亡受到明显抑制.多奈哌齐预处理无保护作用.免疫印迹表明他克林可显著抑制Bax的表达,同时还可促进Bcl-2的表达.多奈哌齐和他克林预处理对STS诱导的HeLa细胞损伤无明显的保护作用.结论他克林的抗凋亡作用与AChE抑制作用似乎没有明显关联.他克林对STS损伤的保护作用有细胞选择性.
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