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EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR IN U937 FOAM CELLS AND THE INHIBITORY EFFECT OF DRUGS
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 2
- DOI:
- 10.3321/j.issn:0513-4870.2002.02.002
- Key Word:
- 泡沫细胞;血管内皮生长因子;酶联免疫吸附试验;免疫组织化学;原位杂交;丹酚酸B;银杏叶提取物
Abstract： 目的研究U937泡沫细胞中血管内皮生长因子(VEGF)表达以及丹酚酸B和银杏叶提取物对其的抑制作用.方法U937细胞与80 mg·L-1氧化型低密度脂蛋白(OX-LDL)孵育48 h,建立U937泡沫细胞模型.用基础酶联免疫吸附试验(ELISA)检测各组培养基中VEGF蛋白分泌含量,用免疫组织化学法检测VEGF蛋白表达,用原位杂交法检测VEGF mRNA水平.给予不同浓度丹酚酸B和银杏叶提取物,观察VEGF表达的变化.结果培养的U937泡沫细胞培养液中VEGF含量和细胞中VEGF表达均明显高于U937正常细胞.丹酚酸B和银杏叶提取物加入后阳性反应明显减弱.结论泡沫细胞中VEGF呈高表达、含量明显增加,而丹酚酸B和银杏叶提取物可明显降低其含量.
- [1]K, K?se,P, Do?an,M, A??io?lu,etc.In vitro antioxidant effect of Ginkgo biloba extract (EGb 761) on lipoperoxidation induced by hydrogen peroxide in erythrocytes of Beh?et's patients.[J].1997,75(3).
- [2]M A, Ramos,M, Kuzuya,T, Esaki,etc.Induction of macrophage VEGF in response to oxidized LDL and VEGF accumulation in human atherosclerotic lesions.[J].1998,18(7).
- [3]Mayumi Inoue,Hiroshi Itoh,Makiko Ueda,etc.Vascular Endothelial Growth Factor (VEGF) Expression in Human Coronary Atherosclerotic LesionsPossible Pathophysiological Significance of VEGF in Progression of Atherosclerosis[J].1998,98(20).
- [4]J P, Roncin,F, Schwartz,P, D'Arbigny.EGb 761 in control of acute mountain sickness and vascular reactivity to cold exposure.[J].1996,67(5).
- [5]张黎,芮耀诚,邱彦,等.溶血磷脂酰胆碱对ECV304细胞中血管内皮生长因子表达的影响[J].2001,(4).
- [6]Bryan Williams,Anne Quinn Baker,Barbara Gallacher,etc.Angiotensin II Increases Vascular Permeability Factor Gene Expression by Human Vascular Smooth Muscle Cells[J].1995,25(5).
- [7]Y J, Wu,C Y, Hong,S J, Lin,etc.Increase of vitamin E content in LDL and reduction of atherosclerosis in cholesterol-fed rabbits by a water-soluble antioxidant-rich fraction of Salvia miltiorrhiza.[J].1998,18(3).
- [8]Napoleone Ferrara,William J. Henzel.Pituitary follicular cells secrete a novel heparin-binding growth factor specific for vascular endothelial cells[J].1989,161(2).
- [9]李全忠,杨永宗,易光辉.U937泡沫细胞模型的建立[J].1999,(2).
- [10]Smith, P. F.,Maclennan, K.,Darlington, C. L..The neuroprotective properties of the Ginkgo biloba leaf: a review of the possible relationship to platelet-activating factor (PAF)[J].1996,50(3).
- [11]Li J,Perrella MA,Yet SF,etc.Induction of vascular endothelial growth factor gene expression by interleukin-1 beta in rat aortic smooth muscle cells.[J].1995,270(1).
- [12]Michael Aviram.Modified forms of low density lipoprotein and atherosclerosis[J].1993,98(1).
- [13]O J, de Boer,A C, van der Wal,P, Teeling,etc.Leucocyte recruitment in rupture prone regions of lipid-rich plaques: a prominent role for neovascularization?[J].1999,41(2).