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EFFECTS OF CLONIDINE ON MYOCARDIAL Gsα mRNA EXPRESSION AFTER SCALDS IN RATS
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0513-4870.2002.01.005
- Key Word:
- 可乐定;烧伤;信使RNA;腺苷酸环化酶;环腺苷一磷酸
Abstract： 目的研究可乐定对严重烫伤大鼠心肌Gsα mRNA表达的影响.方法将大鼠置于95℃水浴中烫10 s,造成背部皮肤30%体表面积III度烫伤.分别用dot blotting杂交,原位杂交、放射免疫分析及间接方法测定心肌Gsα mRNA表达水平、cAMP生成量与adenyl cyclase (AC)活性.结果烫伤后3 h心肌Gsα mRNA表达水平明显减少,0.3,1.0,3.0 mg·kg-1可乐定(ip)可增加烫伤后心肌Gsα mRNA表达,表达相对量与可乐定剂量呈正相关.I1-咪唑啉受体拮抗剂efaroxan可部分逆转可乐定增加烫伤后心肌Gsα mRNA表达的作用,减少量与efaroxan剂量呈显著正相关.AC和cAMP的变化与Gsα mRNA类似.结论可乐定可增加烫伤后早期大鼠心肌Gsα mRNA表达、AC活性和cAMP产生.
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