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EFFECTS OF CHRONIC EXPOSURE TO BETA-AMYLOID PEPTIDE25-35 ON VOLTAGE-GATED POTASSIUM OUTWARD CURRENT IN CULTURED RAT HIPPOCAMPAL NEURONS
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 12
- DOI:
- 10.3321/j.issn:0513-4870.2001.12.005
- Key Word:
- β-淀粉样肽(25-35);钾通道;膜片钳技术;海马神经元
Abstract： 目的研究慢性孵育β-淀粉样肽(25-35)(β-AP25-35)对海马神经元上瞬时外向钾电流(IA)和延迟整流钾电流(IK)的影响.方法在培养的大鼠海马神经元上用膜片钳全细胞记录钾通道电流.结果β-AP25-35 3 μmol·L-1孵育细胞24 h,IK电流幅度增加(44.3 ±5.4)%,电流密度由(30.4±6.4) pA·PF-1增加至(43.8±4.7) pA·PF -1;β-AP25-35 10 μmol·L-1孵育12 h,IK电流幅度增加(69.8±4.1)%,电流密度增加至(51.6±7.9) pA·PF-1,呈浓度依赖性;β -AP25-35引起的IK增加对TEA 5 mmol·L-1敏感;β-AP25-35 上调IK的作用主要发生在β-AP25-35用药后48 h内.β-AP25-35对 IA无显著性影响.结论β-AP25-35选择性地增加海马神经元上IK,这一作用可能与β-AP的神经毒性有关.
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