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INFLUENCES OF MELATONIN ON THE GROWTH OF HELA CELLS
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 9
- DOI:
- 10.3321/j.issn:0513-4870.2001.09.001
- Key Word:
- 褪黑激素;HeLa细胞;细胞周期
Abstract： 目的研究褪黑激素对体外培养的人宫颈癌(HeLa)细胞活性的影响.方法 MTT测定褪黑激素的抗肿瘤活性;电镜观察药物对肿瘤细胞形态学的影响;流式细胞仪检测药物对肿瘤细胞周期的影响.结果褪黑激素高浓度(2 mmol*L-1)可抑制HeLa细胞的生长,使细胞倍增时间TD延长 ,细胞杀伤率达61.0%.用此浓度作用3 d,可使S期细胞的比例减少,细胞形态学发生非特异性改变,有较多的坏死细胞和少量凋亡细胞.结论高浓度褪黑激素抑制HeLa细胞增殖,可能与抑制细胞的S期有关.
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