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THE EXPRESSION LEVELS OF GIRK1 AND IRK1 IN MEMORY DEFICIT RATS
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 7
- DOI:
- 10.3321/j.issn:0513-4870.2001.07.002
- Key Word:
- β淀粉样肽;GIRK1;IRK1;记忆
Abstract： 目的 研究大鼠侧脑室注射聚集态β淀粉样肽25-35(β-AP25-35)后不同时间,大脑皮层GIRK1和IRK1的表达.方法 通过Morris水迷宫实验测量大鼠空间学习记忆能力;用RT-PCR方法 测定GIRK1和IRK1的相对表达水平.结果 在Morris水迷宫实验中,第4,5,8及10次训练时,β-AP注射组的潜伏期比假手术组明显延长.β-AP注射后1 d及3 d组和假手术组相比,GIRK1的表达明显降低,17 d后与假手术组相同.IRK1的表达无明显改变.结论 大鼠侧脑室注射聚集态β-AP25-35可引起空间学习记忆能力和钾通道表达改变.β淀粉样肽导致的GIRK1表达抑制可能与脑功能异常有关.
- [1]TOSHITAKA NABESHIMA,ATSUMI NITTA.Memory Impairment and Neuronal Dysfunction Induced by β-Amyloid Protein in Rats[J].1994,174(3).
- [2]R, Morris.Developments of a water-maze procedure for studying spatial learning in the rat.[J].1984,11(1).
- [3]Karschin C,Stuhmer W,Karschin A,etc.IRK(1-3) and GIRK(1-4) inwardly rectifying K+ channel mRNAs are differentially expressed in the adult rat brain.[J].1996,16(11).
- [4]Hirakura Y.,Hirashima N.,Suzuki T.,etc.Membrane perturbation by the neurotoxic Alzheimer amyloid fragment beta25-35 requires aggregation and beta-sheet formation[J].1998,46(4).
- [5]Atsumi Nitta,Akio Itoh,Takaaki Hasegawa,etc.β-Amyloid protein-induced Alzheimer's disease animal model[J].1994,170(1).
- [6]R, Etcheberrigaray,E, Ito,K, Oka,etc.Potassium channel dysfunction in fibroblasts identifies patients with Alzheimer disease.[J].1993,90(17).