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POSSIBLE MECHANISM OF THE PROTECTIVE EFFECTS OF GINSENOSIDE Rg1 ON APOPTOSIS IN PC12 CELLS
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0513-4870.2001.06.003
- Key Word:
- 人参皂苷;多巴胺;PC12细胞;细胞凋亡
Abstract： 目的探讨人参皂苷Rg1对多巴胺诱导PC12细胞凋亡的保护作用及其机制.方法用流式细胞仪检测PC12细胞的凋亡率及Bcl-2和Bax蛋白表达率,RT-PCR分析bcl-2和bax mRNA表达水平,荧光分光光度计法检测CPP32活力.结果经10 μmol*L-1 Rg1预处理后,由多巴胺诱导的PC12细胞凋亡受到明显的抑制.同时,Bcl-2蛋白和mRNA表达增加,Bax蛋白和mRNA表达减少,CPP32活力明显下降.结论 Rg1可抑制多巴胺诱导的PC12细胞凋亡,其作用机制可能通过调节Bcl-2与Bax蛋白的比值和抑制CPP32的激活而起作用.
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