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INHIBITORY EFFECTS OF HYDROCORTISONE ON HUMAN POLYMORPHONUCLEAR LEUKOCYTE ADHESION TO HUMAN SYNOVIAL CELL
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0513-4870.2001.06.001
- Key Word:
- 氢化可的松;人滑膜细胞;中性粒细胞;粘附;核因子κB(NF-κB)
Abstract： 目的研究氢化可的松对正常人中性粒细胞(PMN)与滑膜细胞(HSC)粘附的作用及其机理.方法 MTT比色法检测PMN与HSC的粘附,Cell-ELISA和RT-PCR法检测HSC粘附分子的表达,EMSA研究核转录因子NF-κB的活化.结果氢化可的松可显著抑制50 U*mL-1 rhTNF-α与IL-1β刺激的HSC与PMN的粘附;显著抑制HSC表面VCAM-1的表达及VCAM-1 mRNA表达,但对ICAM-1 mRNA的表达无显著影响;同时对TNF-α诱导的NF-κB活化有显著抑制作用.结论氢化可的松显著抑制PMN与HSC的粘附,其作用机理可能是通过抑制NF-κB的活化,进而抑制滑膜细胞中VCAM-1 mRNA及蛋白表达而实现的.
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