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EFFECT OF HEPARINOID DERIVED FROM PORCINE DUODENUM ON THE PROLIFERATION OF CULTURED SMOOTH MUSCLE CELLS
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 11
- DOI:
- 10.3321/j.issn:0513-4870.2000.11.004
- Key Word:
- 动脉粥样硬化;血管平滑肌细胞;类肝素;原癌基因
Abstract： 目的研究类肝素对平滑肌细胞增殖的影响及其机制.方法分别以结晶紫和MTT染色、透射电镜及流式细胞仪等方法,观察类肝素对平滑肌细胞增殖,细胞形态,细胞内α-肌动蛋白含量及原癌基因c-myc,c-fos表达的影响.结果类肝素0.2,0.4及0.8 mg·mL-1可以抑制10%小牛血清所致平滑肌细胞增殖,类肝素0.8 mg·mL-1可以使细胞维持类似收缩型的形态学表现,使细胞内α-actin含量增加,c-myc, c-fos原癌基因表达减少.结论类肝素可以抑制平滑肌细胞增殖,其机制可能与抑制细胞表型转化,抑制细胞内原癌基因表达有关.
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