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EXPRESSION OF FAS AND FASL GENES IN BRAIN AND EFFECT OF FLUNARIZINE ON THESE EXPRESSIONS IN RATS AFTER GLOBAL ISCHEMIA-REPERFUSION
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 11
- DOI:
- 10.3321/j.issn:0513-4870.2000.11.003
- Key Word:
- 脑缺血;Fas受体;FasL配体;基因;氟桂嗪;细胞凋亡
Abstract： 目的观察全脑缺血再灌流后Fas和FasL基因在脑内表达的变化及氟桂嗪的影响.方法用大鼠4血管结扎全脑缺血30 min再灌流模型和免疫组织化学染色法.结果脑缺血再灌流6 h在皮质及海马区即出现Fas表达阳性细胞,24～48 h时达高峰;FasL则在12 h后出现,48～72 h时达到高峰.ip氟桂嗪10 mg.kg-1和20 mg.kg-1则Fas和FasL的表达明显低于不给药的缺血再灌流对照组,并呈剂量依赖性.结论脑缺血再灌流可诱导促凋亡基因Fas和FasL在缺血易感部位表达,钙拮抗剂氟桂嗪可抑制其表达.
- [1]Y, Li,M, Chopp,N, Jiang,etc.Temporal profile of in situ DNA fragmentation after transient middle cerebral artery occlusion in the rat.[J].1995,15(3).
- [2]P A, Negulescu,N, Shastri,M D, Cahalan.Intracellular calcium dependence of gene expression in single T lymphocytes.[J].1994,91(7).
- [3]Tomohiro Matsuyama,Ryuji Hata,Yoshihiro Yamamoto,etc.Localization of Fas antigen mRNA induced in postischemic murine forebrain by in situ hybridization[J].1995,34(1).
- [4]M, Enari,H, Hug,S, Nagata.Involvement of an ICE-like protease in Fas-mediated apoptosis.[J].1995,375(6526).
- [5]Tomohiro Matsuyama,Ryuji Hata,Masafumi Tagaya,etc.Fas antigen mRNA induction in postischemic murine brain[J].1994,657(1-2).
- [6]Naoto Itoh,Shin Yonehara,Ai Ishii,etc.The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis[J].1991,66(2).
- [7]J L, Cleveland,J N, Ihle.Contenders in FasL/TNF death signaling.[J].1995,81(4).
- [8]WILLIAM,PULSINELLI JAMES.A New Model of Bilateral Hemispheric Ischemia in the Unanesthetized Rat[J].1979,10(3).
- [9]PierluigiNicotera,AnnaD.Rossi.Nuclear Ca2+: physiological regulation and role in apoptosis[J].1994,135(1).
- [10]F, Leith?user,J, Dhein,G, Mechtersheimer,etc.Constitutive and induced expression of APO-1, a new member of the nerve growth factor/tumor necrosis factor receptor superfamily, in normal and neoplastic cells.[J].1993,69.
- [11]Sei, Y.,Von Lubitz, D. K. J. E.,Basile, A. S.,etc.Internucleosomal DNA fragmentation in gerbil hippocampus following forebrain ischemia[J].1994,171(1).