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EFFECTS OF ISOPRENALINE ON APOPTOSIS RELATED GENE EXPRESSION IN RAT MYOCARDIUM CELLS
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 11
- DOI:
- 10.3321/j.issn:0513-4870.2000.11.001
- Key Word:
- 急性心肌缺血;细胞凋亡相关基因;异丙肾上腺素;苦豆碱;普萘洛尔
Abstract： 目的研究异丙肾上腺素(isoprenaline, Iso)诱导大鼠急性心肌缺血与心肌细胞凋亡相关基因表达的关系.方法 sc大剂量Iso建立大鼠急性心肌缺血模型.RT-PCR法检测凋亡相关基因Fas,CPP32,Bcl-2和Bax在心肌的表达.并用同样方法,观察苦豆碱(aloperine, Alo, 20 m·kg-1)和普萘洛尔(propranolol, Prop, 2 mg·kg-1)对急性心肌缺血大鼠心肌细胞凋亡相关基因表达的影响.结果急性心肌缺血模型大鼠心肌的Fas,CPP32和Bcl-2基因表达显著增加,Bax变化不明显;Alo和Prop能降低缺血心肌中Fas和CPP32基因的表达,增加Bcl-2基因的表达,且Prop对Bax表达有下调作用.结论 sc大剂量Iso可诱导大鼠心肌细胞凋亡相关基因异常表达;Alo和Prop对该模型大鼠心肌相应基因的异常表达亦有影响.
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