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IMPROVING EFFECT OF NGF BY INTRAHIPPOCAMPAL INJECTION ON AGING-RELATED MEMORY IMPAIRMENT
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 10
- DOI:
- 10.3321/j.issn:0513-4870.2000.10.003
- Key Word:
- 神经生长因子(NGF);记忆;突触体;3H-Leu参入
Abstract： 目的探讨神经生长因子(NGF)改善衰老性记忆障碍的机制.方法采用开场行为和一次性被动回避反应模型,观察海马内微量注射NGF对衰老小鼠自发活动和记忆巩固过程的影响, 同时用Ca2+荧光探针Fura-2/AM和AR-CM-MIC阳离子测定系统测定海马突触体游离钙水平,以3H-Leu为标记物测定海马突触体总蛋白的合成量.结果 NGF使衰老小鼠在新异环境中的自发活动和探究行为明显增多,并显著延长电击后24 h的步入潜伏期(STL); NGF显著降低衰老小鼠海马突触体内的高钙水平,并使海马突触体蛋白质的3H-Leu参入量显著增加.结论 NGF改善衰老性记忆障碍与其降低海马突触体内高钙水平,并由此促进海马突触体蛋白质的合成有关.
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