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STUDY ON POTASSIUM CHANNELS OF RAT INTRAPULMONARY ARTERY SMOOTH MUSCLE CELLS
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 9
- DOI:
- 10.3321/j.issn:0513-4870.2000.09.004
- Key Word:
- 肺内动脉;膜片钳;全细胞记录;钙激活钾电流;延迟整流钾电流
Abstract： 目的研究大鼠肺内动脉平滑肌细胞钾电流.方法用急性酶解分离法得到单个大鼠肺内动脉平滑肌细胞,采用膜片钳全细胞记录方式研究钾通道特性.结果将平滑肌细胞钳制在-70 mV,给予-70～50 mV的斜坡刺激,时间为600 ms.可引出一随电压逐渐增大的电流,在+50 mV时其值为359±31 pA.细胞内用CsCl取代KCl后,该电流几乎完全消失;细胞外用无钙台氏液灌流,电极内液用高浓度的EGTA时,电流可被抑制50%±1%;细胞外给予特异性钙激活钾通道阻断剂TEA和延迟整流钾通道阻断剂4-AP均可使该电流明显下降.结论大鼠肺内动脉平滑肌细胞的钾电流主要由钙激活钾电流和延迟整流钾电流组成.
- [1]徐文洪,李婉,王晓良.兔血管平滑肌延迟整流钾通道研究及其与克隆Kv1.5通道的电生理特性比较[J].1998,(1).
- [2]D. K. Mistry,C. J. Garland.Nitric oxide (NO)-induced activation of large conductance Ca2+-dependent K+ channels (BKCa) in smooth muscle cells isolated from the rat mesenteric artery[J].1998,124(6).
- [3]Standen NB,Quayle JM.K+ channel modulation in arterial smooth muscle.[J].1998,164(4).
- [4]S L, Archer,J M, Huang,H L, Reeve,etc.Differential distribution of electrophysiologically distinct myocytes in conduit and resistance arteries determines their response to nitric oxide and hypoxia.[J].1996,78(3).