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PROTECTIVE ACTION OF THE SPECIFIC P38 MAPK INHIBITOR SB203580 IN CEREBELLAR GRANULE NEURONS OF RAT PUPS
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 7
- DOI:
- 10.3321/j.issn:0513-4870.2000.07.005
- Key Word:
- 特异性p38MAPK抑制剂SB203580;凋亡;小脑颗粒神经元;蛋白激酶;磷脂酰肌醇-3-激酶
Abstract： 目的研究p38丝裂原激活蛋白激酶(MAPK)选择性抑制剂SB203580对乳鼠小脑颗粒神经元凋亡的保护作用.方法 SD乳鼠小脑颗粒神经元培养,琼脂糖凝胶电泳,SAPK/JNK分析试剂盒作激酶分析.结果 PI-3-K的特异性抑制剂LY294002诱导小脑颗粒神经元凋亡,但SB203580通过抑制细胞凋亡而促进小脑颗粒神经元的存活,且有浓度依赖性.LY294002诱导凋亡的颗粒神经元中c-Jun的表达量和磷酸化水平均升高,JNK被激活.但是,当小脑颗粒神经元生长在含SB203580的高钾培养基中,c-Jun的表达量、磷酸化水平和JNK的活性都明显的降低.结论 SB203580通过抑制JNK的活性,降低c-Jun的表达和磷酸化水平,对小脑颗粒神经元产生保护作用.
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