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CONSTRUCTION OF BAXα HIGH EXPRESSING PC-12 CELL LINE AND THE MECHANISMS OF (-) CLAUSENAMIDE IN INHIBITING APOPTOSIS
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0513-4870.2000.06.002
- Key Word:
- 细胞凋亡;基因转化;重组DNA;遗传载体;bax基因;黄皮酰胺
Abstract： 目的用Bax α高表达的PC12细胞研究了(-)黄皮酰胺的抗细胞凋亡作用.方法先将Bax α cDNA从原核载体pBluescript SK克隆到真核载体pcDNA3.用脂质体转染的方法将pcDNA3-Bax α导入PC12细胞.以6-羟基多巴胺(6-OHDA)诱导Bax α高表达PC12细胞的凋亡.结果 (-)黄皮酰胺(0.1～10 μmol·L-1)可使细胞凋亡率显著降低.结论 (-)黄皮酰胺可以抑制细胞凋亡,对神经退行性病变有一定应用前景.
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