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DIFFERENTIATION EFFECT OF A RETINOIC ACID DERIVATIVE R8607 ON HUMAN ACUTE PREMYELOCYTIC LEUKEMIA NB4 CELL LINE AND ITS MECHANISM
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 5
- DOI:
- 10.3321/j.issn:0513-4870.2000.05.003
- Key Word:
- 维甲类化合物;急性早幼粒白血病;分化诱导;维甲酸受体;细胞周期
Abstract： 目的研究维甲类化合物R8607对人急性早幼粒白血病NB4细胞的分化诱导作用及其作用机理.方法采用NBT还原能力和细胞形态的方法观察R8607对NB4细胞功能和形态的分化作用,采用竞争性结合的方法测定R8607和维甲酸受体的结合能力并用流式细胞术观察其对细胞周期移行的影响.结果 R8607有抑制NB4细胞的生长并能使细胞的功能和形态发生分化的作用; 对维甲酸受体的3个亚型α,β,γ均有结合作用并将NB4细胞周期移行阻断在G1期.结论 R8607通过维甲酸受体的调节通路将NB4细胞阻断在G1期并诱导细胞发生功能和形态的分化.
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