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EFFECTS OF GENISTEIN ON GROWTH AND DIFFERENTIATION OF NB4 HUMAN ACUTE PROMYELOCYTIC LEUKEMIA CELLS
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 4
- DOI:
- 10.3321/j.issn:0513-4870.2000.04.004
- Key Word:
- 4′,5′,7-三羟基异黄酮;白血病细胞;分化;凋亡
Abstract： 目的:研究4′,5′,7-三羟基异黄酮(genistein)对NB4白血病细胞生长和分化的影响,揭示其诱导细胞凋亡作用.方法:分别用NBT还原力分析、DNA电泳分析观察药物诱导分化和凋亡作用;Northern杂交分析和质粒DNA断裂分析检测bcl-2基因表达和DNA拓扑异构酶II活性.结果:Genistein可明显抑制NB4细胞生长,并致细胞大量死亡.在浓度高于40 μmol.L-1时,可诱导典型的凋亡形态,同时,细胞bcl-2基因表达受到抑制.Genistein可促进DNA拓扑异构酶II介导的DNA断裂,但诱导NB4细胞分化作用较弱.结论:Genistein通过诱导细胞凋亡来杀伤和抑制NB4细胞的生长,其机制与抑制DNA拓扑异构酶II活性有关.
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