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ESTABLISHMENT OF A MODEL FOR ADHESION OF POLYMORPHONUCLEAR LEUKOCYTE TO SYNOVIAL CELL
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 2
- DOI:
- 10.3321/j.issn:0513-4870.2000.02.005
- Key Word:
- MTT比色法;嗜中性粒细胞;大鼠关节滑膜细胞
Abstract： 目的:建立大鼠中性粒细胞(PMN)与滑膜细胞(RSC)的粘附模型.方法:MTT比色法.结果:500U·mL-1rhTNF-α与IL-1刺激RSC12h对PMN有明显的粘附作用,MTT-PMN细胞数与540nm吸光度值有良好的线形相关性;氢化考的松(hydrocortisone,Hy)和美洛昔康(meloxicam,Melo)均可抑制PMN与500U·mL-1rhTNF-α/或IL-1诱导的RSC粘附,提示Hy和Melo治疗类风湿性关节炎(RA)可能与其抑制PMN与RSC粘附作用有关.结论:以MTT比色法为基础建立的检测大鼠PMN与RSC的粘附模型,可用于PMN与RSC粘附抑制剂的筛选.
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