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COMPARATIVE STUDY OF THE TOXIC EFFECT OF N-METHYL-4-PHENYL-1,2,3,6-TETRAHYDROPYRIDINE AND DOPAMINE ON RAT BRAIN MITOCHONDRIA IN VITRO
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0513-4870.2000.01.004
- Key Word:
- 1-甲基;4-苯基1;2;3;6-四氢吡啶;多巴胺;线粒体膜电位;线粒体膜流动性;线粒体呼吸链复合酶;氧自由基;ATP酶
Abstract： 目的:观察1-甲基,4-苯基1,2,3,6-四氢吡啶(MPTP)和多巴胺对大鼠脑线粒体损伤作用.方法:大鼠脑线粒体与MPTP和多巴胺温孵后测定膜流动性、膜电位、线粒体肿胀、总ATP酶、呼吸链复合酶活性及线粒体H2O2, 和丙二醛(MDA)含量.结果:二者可降低线粒体膜流动性,MPTP损伤线粒体膜电位,对线粒体肿胀、总ATP酶活性无影响;多巴胺不改变膜电位,却抑制总ATP酶活性,导致线粒体肿胀.二者均增加线粒体中MDA含量.多巴胺使H2O2和生成增加,MPTP仅增加H2O2生成.多巴胺抑制呼吸链复合酶活性,MPTP略有激活作用.结论:MPTP与多巴胺损伤线粒体作用的不同, 可能与通过不同途径刺激氧自由基生成有关.
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