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RECEPTOR MECHANISM OF ESTROGEN STIMULATING BONE FORMATION IN HUMAN OSTEOBLAST-LIKE CELL LINE TE85
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 8
- DOI:
- 10.3321/j.issn:0513-4870.1999.08.001
- Key Word:
- 雌激素;骨质疏松;成骨细胞;骨钙素;雌激素受体
Abstract： 目的:研究雌激素促进人的类成骨细胞株TE85细胞骨形成的作用.方法:用3H-胸腺嘧啶、3H-脯氨酸参入法分别测定细胞的增殖和胶原合成; 紫外分光光度法测定细胞内骨碱性磷酸酶(ALP)活性; 放免法测定细胞内骨钙素(BGP)含量; 放射配基法测定细胞核雌激素受体结合.结果:雌激素(E2, 0.01～1.0 nmol.L-1)可浓度依赖地刺激TE85细胞的3H-胸腺嘧啶和3H-脯氨酸的参入量; 在相同的时间点和剂量下,可增加成骨细胞内ALP活性和BGP的含量.结论:E2通过增加成骨细胞数量、促进细胞胶原蛋白及ALP和BGP的合成而促进成骨作用,这些作用是通过雌激素受体介导的.
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