You Position: Home > Paper
EFFECT OF OPIATE ON RECEPTOR-MEDIATED ADENYLATE CYCLASE-cAMP SIGNAL SYSTEM IN NG-LNCXiNOS CELL WITH THE STABLE EXPRESSION OF INDUCIBLE NITRIC OXIDE SYNTHASE GENE
( views:141, downloads:8 )
- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 7
- DOI:
- 10.3321/j.issn:0513-4870.1999.07.002
- Key Word:
- δ-阿片受体;吗啡;药物耐受;阿片类依赖;一氧化氮合酶
Abstract： 目的:利用稳定表达诱导型NO合酶的NG-LNCXiNOS细胞,观察阿片类药物对受体介导AC-cAMP系统的影响.方法:竞争性蛋白结合法和完整细胞受体结合实验测定cAMP含量和受体结合力.结果:阿片类药物短时程作用抑制AC活性,受阿片受体介导的、百日咳毒素敏感的G蛋白信号通路调节.阿片类药物长时程作用和纳洛酮急性戒断引起受体脱敏,呈时间和剂量依赖性,伴有受体下调.结论:成功地建立阿片耐受和依赖细胞模型,可用于研究AC-cAMP系统和NO-cGMP系统在阿片耐受和成瘾中的调节.
- [1]N, Namir,J, Polastron,S, Allouche,etc.The delta-opioid receptor in SK-N-BE human neuroblastoma cell line undergoes heterologous desensitization.[J].1997,68.
- [2]Ho, B. Y.,Holmes, B. B.,Zhao, J.,etc.Determination of �-opioid receptors in NG108-15 cells[J].1997,319(1).
- [3]P L, Prather,A W, Tsai,P Y, Law.Mu and delta opioid receptor desensitization in undifferentiated human neuroblastoma SHSY5Y cells.[J].1994,270.
- [4]O K, R?nnekleiv,M A, Bosch,M J, Cunningham,etc.Downregulation of mu-opioid receptor mRNA in the mediobasal hypothalamus of the female guinea pig following morphine treatment.[J].1996,216.
- [5]Zang MW,Peng XX,Hu P,etc.Construction of an inducible nitric-oxide synthase gene transferring vector mediated by retrovirus.[J].1998,(2).
- [6]P Y, Law,H H, Loh.delta-Opioid receptor activates cAMP phosphodiesterase activities in neuroblastoma x glioma NG108-15 hybrid cells.[J].1993,43.
- [7]Beata Buzas,John Rosenberger,Brian M. Cox.Regulation of δ-Opioid Receptor mRNA Levels by Receptor-Mediated and Direct Activation of the Adenylyl Cyclase-Protein Kinase A Pathway[J].1997,68(2).
- [8]Evans, Christopher J.,Keith Jr., Duane E..Cloning of a delta opioid receptor by functional expression.[J].1992,258(5090).
- [9]臧梦维,沈琦,汪青,等.逆转录病毒载体介导诱导型NO合酶在神经细胞中表达[J].1999,(3).
- [10]P Y, Law,C, Bergsbaken.Properties of delta opioid receptor in neuroblastoma NS20Y: receptor activation and neuroblastoma proliferation.[J].1995,272.
- [11]Reisine T,Law SF.Changes in the association of G protein subunits with the cloned mouse delta opioid receptor on agonist stimulation.[J].1997,281(3).
- [12]Brigitte L. Kieffer,Katia Befort,Claire Gaveriaux-Ruff,etc.The δ-Opioid Receptor: Isolation of a cDNA by Expression Cloning and Pharmacological Characterization[J].1992,89(24).
- [13]E, Malatynska,Y, Wang,R J, Knapp,etc.Human delta opioid receptor: a stable cell line for functional studies of opioids.[J].1995,6.