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ARSENIC TRIOXIDE INDUCES PROGRAMMED CELL DEATH OF HUMAN GASTRIC ADENOCARCINOMA SGC7901 CELLS AND DECREASES C-MYC GENE EXPRESSION
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 5
- DOI:
- 10.3321/j.issn:0513-4870.1999.05.004
- Key Word:
- 三氧化二砷;胃腺癌SGC7901细胞;细胞程序化死亡;c-myc基因表达
Abstract： 目的:探讨As2O3对胃腺癌SGC7901细胞系的生物学效应及机制.方法:通过MTT还原法检测As2O3对该细胞系存活率的影响,从光学显微镜形态观察,流式细胞仪分析,DNA凝胶电泳,细胞凋亡原位检测(TUNEL)进行细胞凋亡的检测.半定量RT-PCR检测基因表达.结果:As2O3处理SGC7901细胞后,细胞的存活率明显降低,光学显微镜下可见到明显的凋亡细胞,流式细胞仪测定细胞周期的G1期前有亚2倍体的凋亡峰,DNA凝胶电泳显示出典型的凋亡特征: DNA有规律断裂形成的梯状图谱,细胞凋亡原位检测发现DNA的断裂,并降低细胞c-myc基因的表达.结论:As2O3能诱导人胃腺癌SGC7901细胞程序化死亡并可能通过降低c-myc基因的表达.
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