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EFFECTS OF BUTYLPHTHALIDE ON THE ACTIVITIES OF COMPLEXES OF THE MITOCHONDRIAL RESPIRATORY CHAIN
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 4
- DOI:
- 10.3321/j.issn:0513-4870.1999.04.001
- Key Word:
- 正丁基苯酞;局灶性脑缺血;呼吸链复合酶;培养神经细胞
Abstract： 目的:阐明正丁基苯酞(dl-3-n-butylphthalide, dl-NBP)改善缺血脑能量代谢的机制.方法:用分光光度法测定局灶性脑缺血大鼠脑线粒体呼吸链复合酶I,II,III和IV活性的改变,并观察dl-NBP对这些变化的影响.结果:缺血时复合酶II活性升高,IV的活性显著降低;再灌后, 复合酶I活性升高,II的活性降低.在缺血前10 min给予dl-NBP(5 mg.kg-1或10 mg.kg-1, ip)能逆转缺血-再灌引起的上述复合酶活性改变,尤其是使缺血后急剧降低的复合酶IV活性得到明显提高.同时NBP(d-,l-,dl-)还能逆转低糖低氧造成的原代培养大鼠皮质细胞呼吸链复合酶IV活性降低.结论:NBP能够改善缺血脑内能量状态是直接作用于脑线粒体的结果,而d-NBP起着主要作用.
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