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HYPOXIA/HYPOGLYCEMIA-INDUCED APOPTOSIS OF RAT CORTICAL NEURONS IS PREVENTED BY DL-3-BUTYLPHTHALIDE
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 3
- DOI:
- 10.3321/j.issn:0513-4870.1999.03.005
- Key Word:
- 丁基苯酞;低氧低糖;神经元;细胞凋亡
Abstract： 目的:以原代培养的大鼠胎鼠皮质神经元低氧低糖再复氧为模型,研究丁基苯酞对神经细胞凋亡的抑制作用.方法:用流式细胞术检测DNA含量及凋亡细胞百分率,透射电镜观察细胞形态学变化,DNA琼脂糖凝胶电泳和原位末段标记(TUNEL)检测DNA断裂. 结果:丁基苯酞能减轻细胞核形态的改变,减少DNA断裂和阳性细胞数,使低氧低糖诱导的神经细胞凋亡百分率明显下降,凋亡峰显著降低. 结论:丁基苯酞对低氧低糖诱导的大鼠皮质神经细胞凋亡有抑制作用.
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