Dynamic expression and significance of E-selectin in rat of acute necrotizing pancreatitis induced lung injury

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Author:
SHENG Min(Derpatment of Surgery, Union Hospital, Fujian medical University, Fuzhou 350000, China)
WANG Feng(Derpatment of Surgery, Union Hospital, Fujian medical University, Fuzhou 350000, China)
HUANG He-guang(Derpatment of Surgery, Union Hospital, Fujian medical University, Fuzhou 350000, China)
CHEN Zhi-yao(Derpatment of Surgery, Union Hospital, Fujian medical University, Fuzhou 350000, China)
Journal Title:
Chinese Journal of Pancreatology
Issue:
Volume 12, Issue 05, 2012
DOI:
10.3760/cma.j.issn.1674-1935.2012.05.012
Key Word:
Pancreatitis, acute necrotizing; Lung injury; E-selectin ; Models,animal

Abstract: Objective To investigate the expression and significance of E-selectin in lung tissue of rat of acute necrotizing pancreatitis (ANP)-induced lung injury.Methods Rats were randomly divided into control and ANP groups.ANP model was induced by retrograde injection of sodium taurocholate into the biliary and pancreatic duct.The rats were sacrificed at 3,6,12 and 24 h.Serum amylase,albumin,Ca2+ and lung wet/dry (W/D) weight ratio were determined,and pancreas and lung tissues underwent routine pathological examination and histopathological scores were evaluated.The expression of E-selectin mRNA and protein in lung tissue of rat was measured by real-time fluorescence quantitative RT-PCR and immunohistochemistry.Results In ANP group at 6h,the serum level of amylase,histopathological scores of pancreas and lung tissues,lung W/D weight ratio were (3978 ± 476) U/L,8.22 ± 0.63,( 12.31 ± 1.58,5.21 ± 0.20,which were significantly higher than those in control group [ (843 ± 90)U/L,0.22 ± 0.36,0.13 ± 0.34,4.46 ±0.17,P < 0.05 or < 0.01 ],the serum level of albumin was (12.9 ± 1.0)g/L,which was significantly lower than that in control group [ ( 15.6 ± 0.7 ) g/L,P < 0.01 ],the serum level of Ca2 + at 12 h was ( 2.33 ±0.15) mmoL/L,which was significantly lower than that in control group [(2.57 ± 0.23)mmoL/L,P < 0.01 ].E-selectin was located in the cell membrane and cytoplasm of vascular endothelial cells.Expression of E-seleetin mRuNA and protein in the lung tissue at 6h was significantly higher than those in control group (3.51 ±0.45 vs 0.95 ± 0.16,0.174 ± 0.019 vs 0.060 ± 006,P < 0.01 ).Conclusions Pulmonary expression of E-selectin of vascular endothelial cell is significantly up-regulated in a time dependant manner when ANPinduced ALI occurs; and it is involved in leukocyte mural and extravasation,and can promote lung injury.

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