Expression of galectin 3 and its effects on proliferation and invasion in pancreatic cancer SW1990 cells

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Author:
ZHOU Guo-xiong(Department of Gastroeterology, Affiliated Hospital, Nantong University,Nantong 226001, China)
ZHANG Miao(Department of Gastroeterology, Affiliated Hospital, Nantong University,Nantong 226001, China)
DING Xiao-ling(Department of Gastroeterology, Affiliated Hospital, Nantong University,Nantong 226001, China)
ZHANG Hai-feng(Department of Gastroeterology, Affiliated Hospital, Nantong University,Nantong 226001, China)
CAO Wei(Department of Gastroeterology, Affiliated Hospital, Nantong University,Nantong 226001, China)
JU Li-shuai(Department of Gastroeterology, Affiliated Hospital, Nantong University,Nantong 226001, China)
KUAI Xiao-ling(Department of Gastroeterology, Affiliated Hospital, Nantong University,Nantong 226001, China)
ZHANG Hong(Department of Gastroeterology, Affiliated Hospital, Nantong University,Nantong 226001, China)
XU Zheng-Fu(Department of Gastroeterology, Affiliated Hospital, Nantong University,Nantong 226001, China)
Journal Title:
Chinese Journal of Pancreatology
Issue:
Volume 12, Issue 05, 2012
DOI:
10.3760/cma.j.issn.1674-1935.2012.05.009
Key Word:
Pancreatic neoplasms ; Galectin-3 ; Cell proliferation ; Neoplasm invasiveness

Abstract: Objective To investigate the expression of galectin-3 in pancreatic cancer cell and its effect on the proliferation and invasion of SW1990.Methods Immunocytochemistry and semi-quantitative RT-PCR was used to detect the expression of galectin-3 protein and mRNA in SW1990,PANC1 and ASPC-1 cell lines.Galectin-3 mono-antibody of different concentrations ( 1,2,3,5 μg/ml) was used to treat SW1990 cells for 24,48,72 h,CCK-8 kits were used to detect the proliferation in SW1990 cells; Transwell chamber was used to study the invasion in SW1990.Results Expression of galectin-3 protein and mRNA was present in SW1990,PANC1,and ASPC-1.Galectin 3 mono-antibody inhibited the proliferation and number of invasive cells in a dose and time dependant manner.The inhibitory rates at 72 h were 19.8%,29.9% and 42.7% in 2,3,5 μg/ml galectin 3 mono-antibody groups,the difference among them and control group was statistically significant ( P < 0.05 ).The inhibite rate of permeating membrane cells in 3 μg/ml galectin-3 mono antibody was 37.1%,the difference between this group and control group was statistically significant (P <0.05 ).ConcLusions Galectin-3 is highly expressed in pancreatic cancer cells.Galectin-3 antibody can inhibit the proliferation and migration capability of SW1990 cells.

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