Expression of DNMT3b and microRNA-29b in pancreatic cancer cells and their ralationship

( views:389, downloads:0 )
Author:
WANG Li-hua(Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China)
GAO Jun(Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China)
DU Yi-qi(Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China)
WU Hong-yu(Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China)
JIN Jing(Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China)
LI Shu-de(Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China)
LI Zhao-shen(Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China)
Journal Title:
Chinese Journal of Pancreatology
Issue:
Volume 12, Issue 05, 2012
DOI:
10.3760/cma.j.issn.1674-1935.2012.05.008
Key Word:
Pancreatic neoplasms ; Cell line, tumor; DNA-methyltransferases3b ; MicroRNAs

Abstract: Objective To investigate the expression of DNMT3b mRNA and microRNA-29b (miR-29b) in pancreatic cancer cells and analyze their relationship.Methods Real-time RT-PCR method was used to detect the expression of DNMT3b mRNA and miR-29b in five pancreatic cancer cell lines ( PANC1,BxPC3,CFPAC,AsPC 1,Capan 2 ) and the relationship between DNMT3b mRNA and miR-29b expression was analvzed by pearson linear correlation method.Results The expression of DNMT3b mRNA in PANC1,BxPC3,CFPAC,AsPC 1,Capan 2 were 0.497 ±0.184,0.420 ±0.168,0.439 ±0.217,0.122 ±0.111 and 0.731 ±0.387,while the expression of miR-29b were 0.745 ± 0.596,0.797 ± 1.000,0.464 ± 0.430,1.836 ± 1.623 and 0.216 ± 0.335.The expression of DNMT3b mRNA was negatively correlated with the expression of miR-29b (r =-0.922,P =0.026 ).Conclusions Both DNMT3b and miRNA29b are involved in the carcinogenesis of pancreatic cancer,and they are negatively correlated.

  • [1]Lai EC.MicroRNAs are complementary to 3 UTR sequence mo tifs that mediate negative post-transcriptional regulation.Nat Genet,2002,30:363-364.
  • [2]Milutinovic S,Knox JD,Szyf M.DNA methyltransferase in hibition induces the transcription of the tumor suppressor p21 (WAF1/CIPl/sdil) J Biol Chem,2000,275:6353-6359.
  • [3]Rhee 1,Bachman KE,Park BH,et al.DNMT1 and DNMT3b cooperate to silence genes in human cancer cells.Nature,2002,416:552-556.
  • [4]Fang JY,Lu R,Mikovits JA,et al.Regulation of hMSH2 and hMLH1 expression in the human colon cancer cell line SW1116hy DNA methyltransferase 1.Cancer Lett,2006,233:124-130.
  • [5]Luczak MW,Jagodzinski PP.The role of DNA methylation in cancer development.Folia Histoehem Cytobiol,2006,44:143-145.
  • [6]Song YX,Yue ZY,Wang ZN,et al.MicroRNA-148b is frequently down-regulated in gastric cancer and acts as a tumor suppressor by inhibiting cell proliferation.Mol Cancer,2011,10:1.
  • [7]Fabbri M,Garzon R,Cimmino A,et al.MicroRNA-29 family reverts aberrant methylation in lung cancer by targeting DNA methyltransferases 3A and 3B.Proc Natl Acad Sci USA,2007,104:15805-15810.
  • [8]Garzon R,Liu S,Fabbri M,et al.MicroRNA-29b induces global DNA hypomethylation and tumor suppressor gene reexpression in acute myeloid leukemia by targeting directly DNMT3A and 3b and indirectly DNMT1.Blood,2009,113:6411-6418.
  • [9]Duursma AM,Kedde M,Schrier M,et al.miR-148 targets human DNMT3b protein coding region.RNA,2008,14:872-877.
  • [10]Duursma AM,Kedde M,Schrier M,et al.miR-148 targets human DNMT3b protein coding region.RNA,2008,14:872-877.
  • [11]Takada S,Berezikov E,Choi YL,et al.Potential role of miR-29b in modulation of Dnmt3a and DNMT3b expression in primordial germ cells of female mouse embryos.RNA,2009,15:1507-1514.
  • [12]Ng EK,Tsang WP,Ng SS,et al.MicroRNA-143 targets DNA methyltransferases 3A in colorectal cancer.Br J Cancer,2009,101:699-706.
  • [13]Pan W,Zhu S,Yuan M,et al.MicroRNA-21 and microRNA-148a contribute to DNA hypomethylation in lupus CD4 + T cells by directly and indirectly targeting DNA methyltransferase 1.J Immunol,2010,184:6773-6781.
  • [14]吕赛群,金华君,钱其军.miR-29b对肝癌细胞中甲基化转移酶表达的调控作用.浙江理工大学学报,2011,23:101-105.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn