MicroRNA profiles in pancreatic ductal adenocarcinoma

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Author:
ZHANG Jing(Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China)
ZHAO Chen-yan()
LIU Qing-hua(Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China)
YU Dang-hui(Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China)
CHEN Ying(Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China)
SHI Min(Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China)
NI Can-rong(Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China)
ZHU Ming-hua(Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China)
Journal Title:
Chinese Journal of Pancreatology
Issue:
Volume 12, Issue 05, 2012
DOI:
10.3760/cma.j.issn.1674-1935.2012.05.007
Key Word:
Pancreatic neoplasms; MicroRNA; Microarray analysis; Gene expression profiling

Abstract: Objective To study the differentially expressed microRNA (miRNA) between pancreatic ductal adenocarcinoma (PDAC) and para-cancerous tissues,and determine related target genes.Methods Nine fresh PDAC tumor tissues and 3 adjacent normal pancreatic tissues were collected,then Agilent miRNAmicroarray with 713 miRNA loci was used to identify the differentially expressed miRNA.Real-time PCR method was applied to verify the up-regulated miRNA.TargetScan 5.1 and miRandaV5 software were used to analyze the related target genes.Results miRNA microarray identified 11 PDAC related miRNAs,among them,the expressions of miR-194*,miR-192*,miR-602,miR-194 were up-regulated,while the expressions of miR-139-3p,miR-513a-5p,miR-630,miR-30c-1 *,miR-887,miR-508-5p,miR-516a-5p were down-regulated.The expressions of miR-192,miR-194 and their homolog were verified in 31 PDAC tumor tissues.After software analysis,it was found that target genes of miR-192 were ZEB2,CXCL-2,EEF1A1,ERCC3,and target genes of miR-192 * were DCC,SMAD4,FAS,and target genes of miR-194 included DACHI,IGSF11,PTPN2,RBBP4,while target genes of miR-194 * included CD40LG,CIDEB,FHL1.Conclusions Eleven differentially expressed miRNAs are present in PDC,and they may be involved in the occurrence and development of PDC.

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