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Effects of MMI-166 on proliferation and apoptosis in human pancreatic cancer SW1990 cell

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Author:
No author available
Journal Title:
Chinese Journal of Pancreatology
Issue:
2
DOI:
10.3760/cma.j.issn.1674-1935.2012.02.008
Key Word:
胰腺肿瘤;细胞系,肿瘤;金属蛋白酶类组织抑制剂;细胞增殖;细胞凋亡;Pancreatic neoplasms;Cell line,tumor;Tissue inhibitor of metalloproteinases;Cell proliferation;Apoptosis

Abstract: Objective To investigate the effects of MMI-166 on the proliferation and apoptosis of human pancreatic cancer SW1990 cells.Methods MMI-166 of different concentrations (25,50,100 μg/ml) were used to treat human pancreatic cancer SW1990 cell for 24,48 h.Effect of MMI-166 on cell proliferation was detected by 3- (4,5-dimethyl-2-thiazole) -2-5-biphenly-tetrazole bromide ( MTT ) method and effect on cell apoptosis was tested by Annexin V-PI method and flow cytometry (FCM).Results Twenty-four hours after MMI-166 treatment of different concentrations (25,50,100 μg/ml),the inhibitory rates of the cells were (34.23±3.87)%,(44.81 ±2.01)%,(53.91 ±1.74)%,and the corresponding values were (39.95 ± 1.83) %,( 52.26 ± 3.46 ) %,( 63.20 ± 2.48 ) % at 48 h,which suggested a time-and concentrationdependent manner.The cell's apoptosis rates were (11.19 ±0.47)%,(23.01 ±0.53)%,(28.10 ± 0.52) % at 24 h,and the corresponding values were ( 11.19 ± 0.47 ) %,( 23.01 ± 0.53 ) %,( 28.10 ± 0.52)% at 48 h,which were significantly higher than those in control group [ (0.09 ±0.12)%,P <0.05].Conclusions MMI-166 can inhibit proliferation and induce apoptosis of human pancreatic SW1990 cell in a time- and concentration-dependent manner.

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