Expression of neuronal intermediate filament protein (NF-66) and relationship with benign and malignancy of insulinomas

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Author:
LIU Bei(Department of Gastroenterology, Peking Union Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China)
XIAO Yu()
ZHU Li-ming(Department of Gastroenterology, Peking Union Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China)
QIAO Xin-wei(Department of Gastroenterology, Peking Union Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China)
XIANG Hong-ding()
CHEN Jie()
CHEN Yuan-Jia()
Journal Title:
Chinese Journal of Pancreatology
Issue:
Volume 12, Issue 02, 2012
DOI:
10.3760/cma.j.issn.1674-1935.2012.02.005
Key Word:
Insulinoma;Intermediate filament proteins;Diagnosis, differential;Prognosis

Abstract: Objective To investigate whether neuronal intermediate filament protein(NF-66) could be used as a molecular marker for the determination of malignancy of insulinoma.Methods The expression of NF-66 protein was detected in insulinoma and pana - cancerous tissues and 3 insulinoma cell lines by immunohistochemistry staining. The relationship between the expression of NF-66 protein and the clinicopathological characteristics,survival was analyzed by univariate and multivariate statistical analysis.Results Expression of NF-66 was found in 102(77% ) out of 132 insulinomas and none of 98 paired control (P =4.86 × 10-31 ).NF-66 was highly expressed in 3 insulinoma cell lines.The expression of NF-66 was found in 96 (81%) out of 118 benign tumors (P =0.003),while out of 14 malignant insulinomas,6(43% ) were found to express NF-66 ( P =0.003 ).The expression of NF-66 was significantly associated with tumor size (3 cm as the cut-off point),distant metastasis (38% vs 81%,P =0.013) and distant plus lymph node metastasis (46% vs 81%,P =0.009),respectively.The expression of NF-66 was not correlated with age,gender,recurrence and overall survival.Conclusions Down-regulation of NF-66 was significantly associated with tumor malignancy,suggesting that NF-66 could be a potentially novel molecular bionarker to distinguish malignancy from benign insulinoma.

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