Dignoosis value of serum glypican-3 for pancreas cancer

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Author:
XIAO Ming-bing(Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, China)
XIE Lin(Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, China)
NI Wen-kai(Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, China)
CHEN Bu-you(Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, China)
LU Cui-hua(Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, China)
LI Xiao-yan(Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, China)
JIANG Feng(Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, China)
NI Run-zhou(Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, China)
Journal Title:
Chinese Journal of Pancreatology
Issue:
Volume 12, Issue 02, 2012
DOI:
10.3760/cma.j.issn.1674-1935.2012.02.001
Key Word:
Pancreatic neoplasms;Galectin-3;Time-resolved fluoroimmunoassay;Serum

Abstract: Objective To establish the time-resolved fluoroimmunoassay (TRFIA) method for the detection of serum galectin-3 and investigate the clinical value of serum galectin-3 for the diagnosis of pancreas cancer.Methods Monoclonal anti-human galectin - 3 antibody and biotinylated polyclonal antibody were used to establish the sandwich TRFIA for detection of serum galectin-3.The optimal experimental condition was studied.Serum levels of galectin-3,CEA and CA19-9 in the patients with pancreatic cancer,benign pancreatic mass,pancreatitis,and healthy controls were measured.The diagnostic value of serum galectin-3,CEA and CA19-9 for pancreas cancer was studied.Results The linearity of the TRFIA for detection of serum galectin3 tanged between 0 to 100 μg/L.The within-run CV and between-run CV were ≤6.45% and ≤8.68%,respectively,and the average recovery was 106.6%.The level of serum galectin-3 was 4.93 ( 0.85 ~ 23.80) μg/L in pancreatic cancer group,which were significantly higher than those in benign pancreatic mass [2.83 ( 2.17 ~ 4.06) μg/L ],pancreatitis [ 2.62 (0.55 ~ 9.76 ) μg/L ],and healthy controls group [ 1.88 ( 0.59 ~ 3.94) μg/L] (P <0.05).By using 3.77 μg/L as the cut-off point,the smsitivity,specificity for the diagnosis of pancreatic cancer was 75.5% and 90.9%.The levels of Gal 3 and CEA,CA19-9 was not correlated ( r =0.1321,P =0.3761 ; r =0.0920,P =0.5384).Combined determination of galactin-3 and CEA,CA19-9 levels could increase the diagnostic sensitivity to 92%.Conclusions TRFIA method for the detection of galactin-3 is sensitive and stable.Galectin-3 could be a potentially novel serum tumor marker of pancreatic cancer.

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