Effect on metastasis of pancreatic cancer in mice injected with KAI1 gene in vivo

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Author:
TIAN Hong(Department of Gastroenterology, General Hospital of Shenyang Military Command, Shenyang 110016, China)
GUO Xiao-zhong(Department of Gastroenterology, General Hospital of Shenyang Military Command, Shenyang 110016, China)
XU Jian-hua(Department of Gastroenterology, General Hospital of Shenyang Military Command, Shenyang 110016, China)
CUI Zhong-min(Department of Gastroenterology, General Hospital of Shenyang Military Command, Shenyang 110016, China)
XIA Chun-lian(Department of Gastroenterology, General Hospital of Shenyang Military Command, Shenyang 110016, China)
WANG Di(Department of Gastroenterology, General Hospital of Shenyang Military Command, Shenyang 110016, China)
REN Li-nan(Department of Gastroenterology, General Hospital of Shenyang Military Command, Shenyang 110016, China)
WU Chun-yan(Department of Gastroenterology, General Hospital of Shenyang Military Command, Shenyang 110016, China)
SHAO Xiao-dong(Department of Gastroenterology, General Hospital of Shenyang Military Command, Shenyang 110016, China)
Journal Title:
CHINESE JOURNAL OF PANCREATOLOGY
Issue:
Volume 8, Issue 05, 2008
DOI:
Key Word:
Pancreatic neoplasms;Adenoviruses;Neoplasm metastasis;KAI1 gene

Abstract: Objective To observe the inhibitory effect on metastasis and growth of pancreatic cancer in mice by injection of KAI1 gene in vivo. Methods Pancreatic cancer cell line MiaPaCa Ⅱ was used to construct the nude mice models bearing tumors, then the mice were divided into normal saline group, Ad group and Ad-KAI1 group. Since the 10th days of model construction, the Ad-KAI1 was injected every 7 d and repeated twice, then the tumor size, the weight of liver, lung and their pathologic changes were evaluated. Results The tumor sizes were not significantly different between the three groups. The weight of lung and liver of Ad-KAI1 group was (0.366±0.041) g and (1. 35±0.21) g, respectively; the weight of lung and liver of Ad group was (0.57±0.065) g and (1.58±1.828) g, respectively; the weight of lung and liver of control group was (0.66±0.13)g and (1.95±0.344)g, respectively. The difference between Ad-KAI1 group and control group was significantly different (t = 5.984, P < 0. 05), and there was no significant difference between Ad group and control group (t=1.089, P > 0.05). The number of pulmonary, liver and lymph node metastasis in Ad-KAI1 group was (1±1), (2±1) and (2±2), respectively; in Ad group was (6±2), (5 ±1), (10±2), respectively; in control group was (7±2), (6±2), (11±3), respectively. The difference between Ad-KAI1 group and control group was significantly different (t = 7.44, 4.34, 8. 16, P < 0.05), while the difference between Ad group and control group was not significantly different (t=0.92, 0.64, 0.42, P >0.05). Conclusions KAI1 gene directly injected into tumors of nude mice may inhibit the growth and metastasis of pancreatic cancer.

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