Silencing of NF-κB p65 expression by RNA interference inhibits proliferation and invasion of pancreatic carcinoma cell strain PANC1

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Author:
WANG Dian-zhong(Department of General Surgery, Drum Tower Hospital, Medical School, Nanjing University, Nanjing 210008, China)
XIE Min(Department of General Surgery, Drum Tower Hospital, Medical School, Nanjing University, Nanjing 210008, China)
PAN Yi-ming(Department of General Surgery, Drum Tower Hospital, Medical School, Nanjing University, Nanjing 210008, China)
ZHU Hai-tao(Department of General Surgery, Drum Tower Hospital, Medical School, Nanjing University, Nanjing 210008, China)
Journal Title:
CHINESE JOURNAL OF PANCREATOLOGY
Issue:
Volume 8, Issue 02, 2008
DOI:
Key Word:
Pancreatic neoplasms; RNA interference; NF-κB; ICAM-1

Abstract: Objective To investigate the inhibitory effect of proliferation and invasiveness on PANC1 cell by knockdown of NF-κB P65 expression through RNA interference. Methods Chemically synthesized small interference RNA (siRNA) directed against human NF-κB p65 was transfected into pancreatic carcinoma cell (PANC1) by using cationic liposome LipofectamineTM 2000 as the transfection agent. A non-silencing siRNA was used as the negative control and distilled water was used as the blank control. The tests repeated for six times. The expression of NF-KB p65 and ICAM-1 gone was detected by RT-PCR. The effect of cell proliferation and invasive ability was evaluated by MTF, clony assay, Matrigel invasive tests. Results The relative expression levels of NF-κB p65 mRNA in siRNA, the negative control and the blank control group were 0.227 ±0.045, O. 381 ± 0. 038 and 0. 404 ± 0. 031, respectively; the relative expression levels of ICAM-1mRNA were 0.597 ± 0. 083, 0.983 ± 0.068 and 1. 027 ± 0.098, respectively; the inhibitory rate of the cells at 72h were 18.3%, 2.3% and 0, respectively; the colony forming efficiency were ( 14.1 ± 3.1 ) %, ( 24.5±2.1)% and (27.2 ±2.6)%, respectively; the number of invasive cells were 80.25 ±6.35, 123.83 ±8.80 and 127.68 ± 9.23, respectively; the difference between the siRNA group and two control groups was significant (P < 0. 01 ). Conclusions Down-regulatiag the expression of NF-κB p65 gene by RNAi may inhibit proliferation and invasiveness in pancreatic carcinoma cell strain PANC1.

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