The expression of KAI1 gene by small interfering RNA (siRNA) in human pancreatic cancer cell line T3

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Author:
MI Yan-bin(Department of Gastroenterology, Xijing Hospital, Forth Military Medical University, Xi'an 710032, China)
GUO Xiao-zhong(Department of Gastroenterology, Xijing Hospital, Forth Military Medical University, Xi'an 710032, China)
LIU Feng(Department of Gastroenterology, Xijing Hospital, Forth Military Medical University, Xi'an 710032, China)
XU Jian-hua(Department of Gastroenterology, Xijing Hospital, Forth Military Medical University, Xi'an 710032, China)
TIAN Hong(Department of Gastroenterology, Xijing Hospital, Forth Military Medical University, Xi'an 710032, China)
XIA Chun-lian(Department of Gastroenterology, Xijing Hospital, Forth Military Medical University, Xi'an 710032, China)
WU Kai-chun(Department of Gastroenterology, Xijing Hospital, Forth Military Medical University, Xi'an 710032, China)
FAN Dai-ming(Department of Gastroenterology, Xijing Hospital, Forth Military Medical University, Xi'an 710032, China)
Journal Title:
CHINESE JOURNAL OF PANCREATOLOGY
Issue:
Volume 8, Issue 02, 2008
DOI:
Key Word:
Pancreatic neoplasms; RNA, small interfering; KAI1; Cell line

Abstract: Objective To evaluate the expression of KAII (CD82) gene inhibited by small interfering RNA (siRNA) in human pancreatic cancer cell line T3. Methods Four sequences of siRNA including A, B,C, D were designed, which were based on the KAI1 gene sequence using online RNA interfering designing software and lentivirus vector was built. Then they were used to transfect T3 cells by liposome 2000 and virus titer was determined. Empty vector containing siRNAd1 lentivrus particle ( MOI =5) was also used to infect T3 cells. The expression of CD82 mRNA was detected by real-time PCR. Results The expression of CD82 mRNA in normal control group, empty vector group, A group, B group, C group, D group were 1. 398 ±0.242,1. 311±0.048, 0. 664 + 0. 093, 0. 345 ± 0. 032, 0. 641 ± 0. 049 and 0. 147 ± 0. 049, respectively, the difference between the expression of CD82 mRNA in empty vector group and that of A, B, C, D groups was significant (P<0.01 ). Conclusions RNAi was able to inhibit the expression of KAI1 gene CD82 in human pancreatic cancer cell line T3.

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