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Clinical significance of Plk1 gene expression in pancreatic carcinoma
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PANCREATOLOGY
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1674-1935.2007.01.003
- Key Word:
- 胰腺肿瘤;Plk1;肿瘤细胞,培养的;PANC 1;CFPAC 1
Abstract： 目的 研究胰腺癌组织标本及细胞系中Plk1的表达及临床意义.方法 应用免疫组化EnVision两步法检测33例胰腺癌及其癌旁组织、3例胰腺浆液性囊腺瘤、3例黏液性囊腺瘤、5例慢性胰腺炎的石蜡标本中Plk1的表达.用半定量RT-PCR检测5例新鲜胰腺癌组织及其癌旁组织中Plk1 mRNA的表达.用Western blot检测两株胰腺癌细胞PANC1和CFPAC1中Plk1表达.结果 胰腺癌组织中Plk1的阳性表达率为72.7%(24/33),其表达与胰腺癌患者的年龄、性别、临床分期、组织学分级、淋巴结转移、肿瘤的部位无显著相关(P ＞ 0.05).癌旁组织、慢性胰腺炎和良性胰腺肿瘤组织中Plk1的表达均为阴性.5例新鲜胰腺癌组织中都有Plk1 mRNA的高表达,两株胰腺癌细胞中也有Plk1蛋白的表达.结论 Plk1在胰腺癌中的表达具有一定肿瘤特异性,有可能成为胰腺癌诊治的理想靶标.
- [1]Gekeler V,Kobel M,Dietel M,etc.Polo-like kinase isoform expression is a prognostic factor in ovarian carcinoma.[J].2004,90(4).
- [2]Spankuch B,Knecht R,Kaufmann M,etc.Cancer inhibition in nude mice after systemic application of U6 promoter-driven short hairpin RNAs against PLK1.[J].2004,96(11).
- [3]Piiper A,Elez R,Pliquett U,etc.Tumor regression by combination antisense therapy against Plk1 and Bcl-2.[J].2003,22(1).
- [4]Strebhardt K,Eckerdt F,Yuan J.Polo-like kinases and oncogenesis.[J].2005,24(2).
- [5]Langrehr J,Bahra M,Weichert W,etc.Overexpression of Polo-like kinase 1 is a common and early event in pancreatic cancer.[J].2005,5(2).
- [6]Alberts GF,Winkles JA.Differential regulation of polo-like kinase 1, 2, 3, and 4 gene expression in mammalian cells and tissues.[J].2005,24(2).
- [7]Erikson RL,Liu X.Activation of Cdc2/cyclin B and inhibition of centrosome amplification in cells depleted of Plk1 by siRNA.[J].2002,99(13).