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Macrophage migration inhibitory factor plays a pathogenic role in experimental acute necrotizing pancreatitis
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PANCREATOLOGY
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1674-1935.2006.06.002
- Key Word:
- 胰腺炎,急性坏死性;巨噬细胞游走抑制因子;白介素-10
Abstract： 目的 探讨巨噬细胞移动抑制因子(MIF)在实验性急性坏死性胰腺炎(ANP)发病机制中的作用以及抗炎细胞因子白介素-10(IL-10)对其的影响和意义.方法 92只大鼠随机分为对照组、ANP组和IL-10治疗组.腹腔注射左旋-精氨酸制作ANP模型.治疗组于末次精氨酸注射后第2、5、8 h腹腔内注射IL-10 10 000U.各组再分4 h、12 h、24 h和36 h点.检测血清巨噬细胞移动抑制因子(MIF)水平以及胰腺和肺组织的MIF表达.结果 ANP组MIF在造模后4 h已升至高值,并持续维持在较高水平,波动于(117.82 ± 15.73)μg/L至(120.97 ± 11.24)μg/L,显著高于对照组的血清MIF浓度(P ＜0.01或P ＜0.05);治疗组各时点血清MIF浓度低于ANP组(P ＜0.05).对照组大鼠胰腺组织的外分泌部、肺组织的支气管上皮细胞及肺泡细胞有MIF弱表达,ANP组和治疗组胰腺及肺组织MIF阳性细胞数增加、染色明显增强.结论 (1)MIF水平在ANP早期明显升高,ANP时肺及胰腺组织中MIF表达增强,提示MIF参与了大鼠ANP及肺损伤的发病;(2)抗炎细胞因子IL-10对血清及胰腺和肺组织的MIF水平有抑制作用,IL-10可能减轻实验性ANP的胰腺及肺的损害,对胰腺及肺组织有一定保护作用.
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