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Expression of tumor necrosis factor receptor p55/p75 and its significance in pathogenesis of acute pancreatitis
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PANCREATOLOGY
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1674-1935.2005.03.004
- Key Word:
- 胰腺炎;肿瘤坏死因子;受体
Abstract： 目的探讨肿瘤坏死因子受体p55/p75(TNFR-p55/TNFR-p75)在实验性急性水肿型胰腺炎(AEP)发病机制中的作用.方法 36只雄性SD大鼠随机分为对照组与AEP组.AEP组采用雨蛙肽(20 μg/kg体重)腹腔注射,每小时1次,共4次建立大鼠急性水肿型胰腺炎模型,对照组仅给予腹腔注射生理盐水.两组大鼠分别在造模后3、6、12 h处死,收集外周血与胰腺标本.应用ELISA法检测血清TNF-α水平,运用RT-PCR的方法检测外周血单核细胞(PBMC)与胰腺组织中TNFR-p55/TNFR-p75mRNA表达,同时测定血清淀粉酶与病理组织学评分作为急性胰腺炎严重程度的指标.结果 MAP组3个时间点血清淀粉酶、TNF-α水平及胰腺组织炎症评分均显著高于对照组(P < 0.01),在PBMC及胰腺组织中TNFR-p55/TNFR-p75 mRNA的表达均显著上调(P < 0.05).结论 TNF-α是急性胰腺炎病程中的重要细胞因子并通过结合胰腺组织中TNFR-p55、TNFR-p75参与引起胰腺组织损伤;同时TNF-α通过与PBMC中 TNFR-p55/TNFR-p75相互作用导致外周血中白细胞活化,从而影响急性胰腺炎的严重程度.
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