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The Role of Nuclear Factor-kappa B in the Pathogenesis of Severe Acute Pancreatitis-Associted Lung Injury
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PANCREATOLOGY
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1674-1935.2001.01.008
- Key Word:
- 重症急性胰腺炎;肺损伤;NF-κB;TNFα;IL-6;ICAM-1
Abstract： 目的探讨核因子-κB(nuclear factor-kappa B,NF-κB)在重症急性胰腺炎(SAP)肺损伤发病机制中的作用.方法66只雌性Wistar大鼠随机分为正常组、SAP组、PDTC(二硫代氨基甲酸吡咯烷)预处理组三组.SAP模型采用5%的牛磺胆酸钠1 ml/kg胰胆管内逆行注射方法建立.PDTC预处理组在建立SAP模型前1 h腹腔内注射PDTC100ml/kg.后两组分别在3、6、12 h三个时相点将动物处死后(各10只),留取肺组织.应用SP免疫组化方法检测SAP肺组织NF-κB表达情况,运用RT-PCR的方法检测肺组织TNFα、IL-6、ICAM-1 mRNA的表达,并测定肺组织湿/干比值作为肺损伤的指标.结果SAP组肺组织湿/干比值除3 h与正常组相比无差异外,其余各组与正常对照组之间有显著性差异(P＜0.05),肺组织内可见NF-κB活化的中性粒细胞浸润,且肺组织TNFα、IL-6、ICAM-1mRNA表达增加.PDTC预处理组肺组织湿/干比值明显降低,NF-κB活化的中性粒细胞明显减少,TNFa、IL-6、ICAM-1 mRNA表达水平显著降低.结论SAP时存在肺损伤,肺组织NF-κB活化并通过促进TNFa、IL-6、ICAM-1 mRNA的表达而参与肺损伤.
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