Screening of differential proteins in early period in rat kidney with complete unilateral ureteral obstruction
- Author:
- No author available
- Journal Title:
- Chinese Journal of Pediatric Surgery
- Issue:
- 9
- DOI:
- 10.3760/cma.j.issn.0253-3006.2012.09.014
- Key Word:
- 输尿管梗阻;蛋白质组;细胞凋亡;肾;Ureteral obstruction;Proteome;Apoptosis;Kidney
Abstract： Objective To screen the differential proteins in early period with complete unilateral ureteral obstruction(UUO).Methods 2 month old SD rats were divided into UUO-12h group and the control group.The total proteins of two groups were divided and analyzed using two-dimensional difference gel electrophoresis.Spots that were differentially expressed were picked and digested with trypsin and subjected to MALDI-TOF MS for protein identification.RT-PCR was conducted to verify the identified ATP5h,PEBP1 mRNA.Results 6 protein spots whose expression levels were significantly increased or decreased more than 1-fold in UUO-12h group were identified.2 up-regulated expressions of proteins identified by peptide mass fingerprinting were PEBP1 and ATP5h.They were also up-regulated expression compared with the control in term of RT-PCR.Conclusions 2 protein expressions of kidney tissue with UUO were confirmed.PEBP1 and ATP5h probably participate in early phase of kidney injury due to their involvement of apoptosis processes.
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