Effect of ginsenoside Rg3 on reversal of multidrug resistance of hepatocellular carcinoma cells bel-7402: an experimental study

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Author:
WEN Huan-lian(Department of Infectious Disease, The First Affiliated Hospital , Guangzhou Medical College, Guangzhou 51000, china)
LI Xi-mei(Department of Infectious Disease, The First Affiliated Hospital , Guangzhou Medical College, Guangzhou 51000, china)
ZENG Wen-ting(Department of Infectious Disease, The First Affiliated Hospital , Guangzhou Medical College, Guangzhou 51000, china)
ZHU Ke-lun(Department of Infectious Disease, The First Affiliated Hospital , Guangzhou Medical College, Guangzhou 51000, china)
Journal Title:
Chinese Journal of Biomedical Engineering
Issue:
Volume 18, Issue 03, 2012
DOI:
10.3760/cma.j.issn.1674-1927.2012.03.006
Key Word:
Ginsenoside Rg3;Drug resistance,neoplasms;Live neoplasms;Multidrug resistance-association proteins

Abstract: Objective To determine the effect of ginsenoside Rg3 on reversal of multidrug resistance of hepatocellular carcinoma (HCC) cells bel-7402.Methods MTT method was employed to determine the inhibitory rate of ginsenoside Rg3 in combination with vincristine(VCR) on human HCC cells bel-7402.The cffcct of ginscnoside Rg3 on MDR1 mRNA cxpression in HCC cells was detected by reverse transcriptase-polymerase chain reaction(RT-PCR).Results The ginsenoside Rg3 of various concentrations ( 10,20,30,40,60 mg/L) combined with VCR(0.02 mg/L) produced longer inhibitory action than either drug alone (all P<0.05,CDI<1).Ginsenoside Rg3 (40.0 mg/L) led to marked reduction in MDR1 mRNA expression[ (79.21±2.23)%,(62.58±2.46%,(45.46±1.13%) ] in HCC cells bel-7402 at various time points (24 h,48 h and 72 h) as compared with control group (all P<0.05).Conclusion Charactcrizcd by inhibiting MDR1 mRNA expression in bel-7402 cells,ginsenoside Rg3 combined with VCR has co-inhibition on reversal of HCC cells bel-7402 resistance to VCR.

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